Recombinant Human DNAJB8, His-tagged

• Cat.No.: DNAJB8-4730H
• Product Overview: Recombinant human DNAJB8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: DNAJB8, also known as dnaJ homolog subfamily B member 8, is superior suppressors of aggregation and toxicity of disease-associated polyglutamine proteins. DNAJB8 is (de)acetylated at two conserved C-terminal lysines that are not involved in substrate binding, but do play a role in suppressing protein aggregation.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
• Molecular Weight: 27.8 kDa (252aa), confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MANYYEVLGV QASASPEDIK KAYRKLALRW HPDKNPDNKE EAEKKFKLVS EAYEVLSDSK KRSLYDRAGC DSWRAGGGAS TPYHSPFDTG YTFRNPEDIF REFFGGLDPF SFEFWDSPFN SDRGGRGHGL RGAFSAGFGE FPAFMEAFSS FNMLGCSGGS HTTFSSTSFG GSSSGSSGFK SVMSSTEMIN GHKVTTKRIV ENGQERVEVE EDGQLKSVTV NGKEQLKWMD SK
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: DNAJB8 DnaJ (Hsp40) homolog, subfamily B, member 8 [ Homo sapiens ]
• Official Symbol: DNAJB8
• Synonyms: DNAJB8; DJ6; MGC33884; DnaJ (Hsp40) homolog, subfamily B, member 8; dnaJ homolog subfamily B member 8; OTTHUMP00000216243; DnaJ homolog, subfamily B, member 8
• Gene ID: 165721
• mRNA Refseq: NM_153330
• Protein Refseq: NP_699161
• MIM: 611337
• UniProt ID: Q8NHS0
• Chromosome Location: 3q21.3
• Function: heat shock protein binding; unfolded protein binding