Recombinant Human DLL1 protein, His-tagged

• Cat.No.: DLL1-2195H
• Product Overview: Recombinant Human DLL1 (Gln18-Gly540) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Delta-like protein 1 (DLL1) is also known as Drosophila Delta homolog 1 (Delta1 or H-Delta-1), which contains one DSL domain and eight EGF-like domains. DLL1 is ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. As for expression, DLL1 is expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney. Furthermore, DLL1 acts as a ligand for Notch receptors. Also, DLL1 can block the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Gln18
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 58.4 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Gln18-Gly540
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: DLL1
• Official Symbol: DLL1
• Synonyms: DLL1; delta-like 1 (Drosophila); delta (Drosophila) like 1; delta-like protein 1; H-Delta-1; drosophila Delta homolog 1; DL1; Delta; DELTA1
• Gene ID: 28514
• mRNA Refseq: NM_005618
• Protein Refseq: NP_005609
• MIM: 606582
• UniProt ID: O00548