Recombinant Human DKKL1 Protein, Full Length, C-10×His tagged

• Cat.No.: DKKL1-34HFL
• Product Overview: Recombinant Human DKKL1 Protein, Full Length, with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

Specification

• Description: The dickkopf protein family interacts with the Wnt signaling pathway and its members are characterized by two conserved cysteine-rich domains. This gene encodes a secreted protein that has low sequence similarity to the dickkopf-3 protein. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Source: Mouse myeloma cell line
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: Monomer
• Bio-activity: Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is 2-6 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 25.3 kDa
  SDS-PAGE: 40 kDa, reducing conditions
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• N-terminal Sequence Analysis: Ala31
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• References: 1. Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
  2. Krupnik, V.E. et al. (1999) Gene 238:301.
  3. Kohn, M.J. et al. (2005) Mol. Reprod. Dev. 71:516.

Gene Information

• Gene Name: DKKL1 dickkopf-like 1 [ Homo sapiens (human) ]
• Official Symbol: DKKL1
• Synonyms: DKKL1; dickkopf-like 1; dickkopf like 1 (soggy); dickkopf-like protein 1; cancer/testis antigen 34; CT34; SGY 1; soggy; soggy-1; protein soggy-1; SGY; SGY1; SGY-1
• Gene ID: 27120
• mRNA Refseq: NM_001197301
• Protein Refseq: NP_001184230
• MIM: 605418
• UniProt ID: Q9UK85