Recombinant Human dihydrolipoamide dehydrogenase Protein, His&Twin Strep tagged

• Cat.No.: DLD-004H
• Product Overview: Recombinant human DLD protein (P09622) (Ala 36-Phe 509) with a Twin strep tag at the N-terminus and a His tag at the C-terminus was expressed in E. coli.

Specification

• Description: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex).The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion.A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A. In monomeric form may have additional moonlighting function as serine protease. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction
• Source: E. coli
• Species: Human
• Tag: N-Twin strep and C-His
• Protein length: 36-509aa
• Molecular Mass: 55.5 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: DLD dihydrolipoamide dehydrogenase [ Homo sapiens (human) ]
• Official Symbol: DLD
• Synonyms: DLD; dihydrolipoamide dehydrogenase; dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2 oxo glutarate complex, branched chain keto acid dehydrogenase complex), GCSL, LAD; dihydrolipoyl dehydrogenase, mitochondrial; DLDH; E3 component of pyruvate dehydrogenase complex; 2 oxo glutarate complex; branched chain keto acid dehydrogenase complex; diaphorase; lipoamide reductase; lipoyl dehydrogenase; lipoamide dehydrogenase; glycine cleavage system L protein; glycine cleavage system protein L; E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex; E3; LAD; GCSL; PHE3
• Gene ID: 1738
• mRNA Refseq: NM_000108
• Protein Refseq: NP_000099
• MIM: 238331
• UniProt ID: P09622