Recombinant Human DGKE protein, His-tagged

• Cat.No.: DGKE-597H
• Product Overview: Recombinant Human DGKE (P52429) aa. (Leu243~Gly502) fused with His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Leu243~Gly502
• Description: Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 32.5kDa; Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
• Purity: >95%
• Characteristic: Isoelectric point is 7.7
  .
• Applications: Cell culture; Activity Assays; In vivo assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl and 5% trehalose.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: DGKE diacylglycerol kinase epsilon [ Homo sapiens (human) ]
• Official Symbol: DGKE
• Synonyms: DGKE; diacylglycerol kinase epsilon; DGK; AHUS7; DAGK5; DAGK6; NPHS7; DAG kinase epsilon; DGK-epsilon; diacylglycerol kinase, epsilon 64kDa; diglyceride kinase epsilon
• Gene ID: 8526
• mRNA Refseq: NM_003647.2
• Protein Refseq: NP_003638.1
• UniProt ID: P52429