Recombinant Human DFFB Protein, Q201-Q338, C-His tagged

• Cat.No.: DFFB-41H
• Product Overview: Recombinant Human DNA fragmentation factor subunit beta/DFFBis produced by E. coli. The target gene encoding Q201-Q338 is expressed with a His tag at the C-terminus.

Specification

• Description: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined.
• Source: E. coli
• Species: Human
• Tag: His
• Protein length: Q201-Q338
• Stability: 12 months from date of despatch
• Storage: Store at ≤ -70 centigrade, stable for 6 months after receipt. Store at ≤ -70 centigrade, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
• Storage Buffer: Supplied as a 0.2 μm filtered solution of PBS, 300mM NaCl, 10%Glycerol, pH7.4

Gene Information

• Gene Name: DFFB DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) [ Homo sapiens (human) ]
• Official Symbol: DFFB
• Synonyms: DFFB; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase); DNA fragmentation factor subunit beta; CAD; CPAN; DFF 40; DFF40; caspase-activated DNase; caspase-activated nuclease; caspase-activated deoxyribonuclease; DNA fragmentation factor 40 kDa subunit; DFF2; DFF-40
• Gene ID: 1677
• mRNA Refseq: NM_004402
• Protein Refseq: NP_004393
• MIM: 601883
• UniProt ID: O76075