Recombinant Human Delta-like 1 (Drosophila), FLAG-tagged

• Cat.No.: DLL1-2116H
• Product Overview: Recombinant Human DLL1 was produced inHEK 293cells. Signal peptide and extracellular domain of recombinant human DLL1 (Delta-like protein 1; Delta1) (aa 1-545) are fused at the C-terminus to a FLAG-tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• Protein Length: 1-545 a.a.
• Description: DLL1 is the human homolog of the Drosophila Notch ligand Delta. Sequence analysis predicted that the 723-aa DLL1 transmembrane protein, which is 88% identical to the mouse DLL1 protein, has a DSL domain followed by 8 tandem EGF-like repeats and a short cytoplasmic C-terminal region. A soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal aa inhibited the differentiation of myeloid progenitors but promoted their expansion. DLL1 blocks the differentiation of progenitor cells into the B cell lineage while promoting the emergence of a population of cells with the characteristics of a T cell/NK cell precursor. DLL1 undergoes proteolytic processing in its extracellular domain by ADAM10 and represents a substrate for several other members of the ADAM family. The soluble 38kDa Delta1 intracellular domain (DICD) that is generated by the presenilin-dependent γ-secretase cleavage is transported into the nucleus and likely plays a role in transcriptional events. Recently it has been shown that DLL1 is essential for postnatal arteriogenesis.
• Purity: ≥90% (SDS-PAGE).
• Formulation: Liquid. 0.2µm-filtered solution in PBS.
• Endotoxin Content: <0.1EU/µg protein (LAL-test).
• Biological Activity: Inhibits adipogenesis of mesenchymal stem cells.
• Stability: Working aliquots are stable for up to 3 months when stored at -20°C.

Gene Information

• Gene Name: DLL1 delta-like 1 (Drosophila) [ Homo sapiens ]
• Synonyms: DLL1; delta-like 1 (Drosophila); DL1; Delta; DELTA1; delta-like protein 1; H-Delta-1; delta-like 1 protein; drosophila Delta homolog 1; delta (Drosophila)-like 1; Delta1
• Gene ID: 28514
• mRNA Refseq: NM_005618
• Protein Refseq: NP_005609
• MIM: 606582
• UniProt ID: O00548
• Chromosome Location: 6q27
• Pathway: Notch signaling pathway
• Function: Notch binding; calcium ion binding; protein binding