Recombinant Human DEFA6 protein, His & GST-tagged

• Cat.No.: DEFA6-1140H
• Product Overview: Recombinant Human DEFA6 aa. (Ala19~Cys99 (Accession # Q01524) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 6, is highly expressed in the secretory granules of Paneth cells of the small intestine, and likely plays a role in host defense of human bowel.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 41.3kDa.
• Protein length: Ala19~Cys99
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 5.9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: DEFA6 defensin alpha 6 [ Homo sapiens (human) ]
• Official Symbol: DEFA6
• Synonyms: DEFA6; defensin alpha 6; DEF6; HD-6; defensin-6; defensin, alpha 6, Paneth cell-specific
• Gene ID: 1671
• mRNA Refseq: NM_001926.3
• Protein Refseq: NP_001917.1
• UniProt ID: Q01524