Recombinant Human DEFA5 protein, His-tagged

• Cat.No.: DEFA5-1963H
• Product Overview: Recombinant Human DEFA5 aa. (Glu20~Arg94) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 15kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Glu20~Arg94
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 92%
• Characteristic: The isoelectric point is 5.9.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage Buffer: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: DEFA5 defensin alpha 5 [ Homo sapiens (human) ]
• Official Symbol: DEFA5
• Synonyms: HSPA (heat shock 70) binding protein, cytoplasmic cochaperone 1; HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1; HSPA binding protein, cytoplasmic cochaperone 1; heat shock protein-binding protein 1; hsp70-interacting protein 1; hsp70-binding protein 1
• Gene ID: 1670
• mRNA Refseq: NM_021010.2
• Protein Refseq: NP_066290.1
• UniProt ID: Q01523

Reviews

08/16/2019

The product's established reputation in the scientific community provided assurance of its reliability.

07/07/2019

The product's innovative features hinted at its potential for advancing our research in uncharted territories.

12/20/2016

We were excited about the product's role in driving future discoveries in our field of study.

Q&As

Is DEFA5 linked to any diseases?

Altered DEFA5 expression has been associated with various gastrointestinal disorders, including inflammatory bowel diseases (IBD).

Are there specific diseases where DEFA5 is implicated?

DEFA5 has been implicated in conditions such as inflammatory bowel diseases (IBD) and other gastrointestinal disorders.

What is the significance of DEFA5?

DEFA5, known as Defensin Alpha 5, is a crucial member of the defensin family, functioning as an antimicrobial peptide.

What role does DEFA5 play in innate immunity?

DEFA5 contributes to innate immunity by directly targeting and inhibiting the growth of pathogenic microorganisms in the gut.

How does DEFA5 function in host defense?

DEFA5 acts as an antimicrobial peptide, defending the gut against bacterial infections and helping maintain a balanced gut microbiota.

Can DEFA5 be a potential therapeutic target?

DEFA5 is being investigated as a potential therapeutic target for conditions involving gut infections and imbalances in the gut microbiota.

What is the primary tissue of expression for DEFA5?

DEFA5 is primarily expressed in Paneth cells located in the small intestine, contributing to innate immune defense in the gut.