Recombinant Human DEAD (Asp-Glu-Ala-Asp) box helicase 56, His-tagged

• Cat.No.: DDX56-275H
• Product Overview: DDX56, 1-547 aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-547 a.a.
• Description: DDX56 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX56 shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. Recombinant human DDX56 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea
• Molecular Mass: 64.0 kDa (570aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEDSEAL GFEHMGLDPR LLQAVTDLGW SRPTLIQEKA IPLALEGKDL LARARTGSGK TAAYAIPMLQ LLLHRKATGP VVEQAVRGLV LVPTKELARQ AQSMIQQLAT YCARDVRVAN VSAAEDSVSQ RAVLMEKPDV VVGTPSRILS HLQQDSLKLR DSLELLVVDE ADLLFSFGFE EELKSLLCHL PRIYQAFLMS ATFNEDVQAL KELILHNPVT LKLQESQLPG PDQLQQFQVV CETEEDKFLL LYALLKLSLI RGKSLLFVNT LERSYRLRLF LEQFSIPTCV LNGELPLRSR CHIISQFNQG FYDCVIATDA EVLGAPVKGK RRGRGPKGDK ASDPEAGVAR GIDFHHVSAV LNFDLPPTPE AYIHRAGRTA RANNPGIVLT FVLPTEQFHL GKIEELLSGE NRGPILLPYQ FRMEEIEGFR YRCRDAMRSV TKQAIREARL KEIKEELLHS EKLKTYFEDN PRDLQLLRHD LPLHPAVVKP HLGHVPDYLV PPALRGLVRP HKKRKKLSSS CRKAKRAKSQ NPLRSFKHKG KKFRPTAKPS
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: DDX56 DEAD (Asp-Glu-Ala-Asp) box helicase 56 [ Homo sapiens ]
• Official Symbol: DDX56
• Synonyms: DDX56; DEAD (Asp-Glu-Ala-Asp) box helicase 56; DEAD (Asp Glu Ala Asp) box polypeptide 56; probable ATP-dependent RNA helicase DDX56; NOH61; nucleolar helicase of 61 kDa; DEAD box protein 21; DEAD box protein 56; DEAD-box RNA helicase; 61-kd nucleolar helicase; putative nucleolar RNA helicase; DEAD (Asp-Glu-Ala-Asp) box polypeptide 56; ATP-dependent 61 kDa nucleolar RNA helicase; DDX21; DDX26
• Gene ID: 54606
• mRNA Refseq: NM_001257189
• Protein Refseq: NP_001244118
• MIM: 608023
• UniProt ID: Q9NY93
• Chromosome Location: 7p13
• Function: ATP binding; ATP-dependent RNA helicase activity; RNA binding; helicase activity; hydrolase activity; nucleotide binding