Recombinant Human DDX39A, His-tagged

• Cat.No.: DDX39A-226H
• Product Overview: DDX39A, 1-249aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-249aa
• Description: DDX39A is a member of the DEAD box protein family. This protein is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Alternatively spliced transcript variants encoding different isoforms have been found. Recombinant human DDX39A protein, fused to His-tag at N-terminus, was expressed in E.coli
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea
• Molecular Mass: 31.0 kDa (274aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSEFMAEQD VENDLLDYDE EEEPQAPQES TPAPPKKDIK GSYVSIHSSG FRDFLLKPEL LRAIVDCGFE HPSEVQHECI PQAILGMDVL CQAKSGMGKT AVFVLATLQQ IEPVNGQVTV LVMCHTRELA FQISKEYERF SKYMPSVKVS VFFGGLSIKK DEEVLKKNCP HVVVGTPGRI LALVRNRSFS LKNVKHFVLD ECDKMLEQLD MRRDVQEIFR LTPHEKQCMM FSATLSKDIR PVCRKFMQDP MEVF
• Purity: >80 % by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: DDX39A DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A [ Homo sapiens ]
• Official Symbol: DDX39A
• Synonyms: DDX39A; DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A; DDX39, DEAD (Asp Glu Ala Asp) box polypeptide 39 , DEAD/H (Asp Glu Ala Asp/His) box polypeptide 39; ATP-dependent RNA helicase DDX39A; BAT1L; DDXL; UAP56 related helicase; 49 kDa; URH49; DEAD box protein 39; nuclear RNA helicase URH49; UAP56-related helicase, 49 kDa; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 39; DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 transcript; nuclear RNA helicase, DECD variant of DEAD box family; BAT1; DDX39; MGC8417; MGC18203
• Gene ID: 10212
• mRNA Refseq: NM_005804
• Protein Refseq: NP_005795
• UniProt ID: O00148
• Chromosome Location: 19p13.12
• Function: ATP binding; ATP-dependent helicase activity; helicase activity; hydrolase activity; nucleic acid binding; nucleotide binding; protein binding