Recombinant Human DDX27 protein, His-tagged

• Cat.No.: DDX27-3516H
• Product Overview: Recombinant Human DDX27 protein(418-767 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 418-767 aa
• AA Sequence: EGDREAIVAALLTRTFTDHVMLFTQTKKQAHRMHILLGLMGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFTMPNTIKHYVHRVGRTARAGRAGRSVSLVGEDERKMLKEIVKAAKAPVKARILPQDVILKFRDKIEKMEKDVYAVLQLEAEEKEMQQSEAQINTAKRLLEKGKEAVVQEPERSWFQTKEERKKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERSQFEILKAQMFAERLAKRNRRAKRARAMPEEEPVRGPAKKQKQGKKSVFDEELTNTSKKALKQYRASPSFEERKQLGLPHQRRGGNFKSKSRYKRRK
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: DDX27 DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 [ Homo sapiens ]
• Official Symbol: DDX27
• Synonyms: DDX27; DEAD (Asp-Glu-Ala-Asp) box polypeptide 27; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 27; probable ATP-dependent RNA helicase DDX27; dJ686N3.1; DRS1; DEAD box protein 27; RNA helicase-like protein; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 27; RHLP; Rrp3p; PP3241; HSPC259; MGC1018; FLJ12917; FLJ20596; FLJ22238; MGC163147; DKFZp667N057
• Gene ID: 55661
• mRNA Refseq: NM_017895
• Protein Refseq: NP_060365
• UniProt ID: Q96GQ7