Recombinant Human DDOST 293 Cell Lysate

• Cat.No.: DDOST-7024HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for dolichyl-diphosphooligosaccharide-protein glycosyltransferase (DDOST) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: DDOST dolichyl-diphosphooligosaccharide--protein glycosyltransferase [ Homo sapiens ]
• Official Symbol: DDOST
• Synonyms: DDOST; dolichyl-diphosphooligosaccharide--protein glycosyltransferase; dolichyl diphosphooligosaccharide protein glycosyltransferase; dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit; KIAA0115; OST; OST48; WBP1; DDOST 48 kDa subunit; advanced glycation endproduct receptor 1; oligosaccharyltransferase 48 kDa subunit; oligosaccharyl transferase 48 kDa subunit; dolichyl-diphosphooligosaccharide-protein glycotransferase; dolichyl-diphosphooligosaccharide-protein glycosyltransferase; AGER1; CDG1R; OKSWcl45; MGC2191
• Gene ID: 1650
• mRNA Refseq: NM_005216
• Protein Refseq: NP_005207
• MIM: 602202
• UniProt ID: P39656
• Chromosome Location: 1p36.1
• Pathway: Advanced glycosylation endproduct receptor signaling, organism-specific biosystem; Asparagine N-linked glycosylation, organism-specific biosystem; Gene Expression, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem
• Function: contributes_to dolichyl-diphosphooligosaccharide-protein glycotransferase activity; contributes_to dolichyl-diphosphooligosaccharide-protein glycotransferase activity; contributes_to oligosaccharyl transferase activity; transferase activity