Recombinant Human DDB2 Protein (Pro166-Met383), His tagged

• Cat.No.: DDB2-1933H
• Product Overview: Recombinant human DDB2 (Pro166-Met383) fused with His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Pro166-Met383
• Description: Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also functions as the substrate recognition module for the DCX (DDB2-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB2-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB2-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB2-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. The DDB2-CUL4-ROC1 complex also ubiquitinates KAT7/HBO1 in response to DNA damage, leading to its degradation: recognizes KAT7/HBO1 following phosphorylation by ATR
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 25.76 kDa
• Purity: >90% as determined by SDS-PAGE
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS, pH 7.5 or lyophilized from PBS, pH 7.5.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: DDB2 damage-specific DNA binding protein 2, 48kDa [ Homo sapiens (human) ]
• Official Symbol: DDB2
• Synonyms: DDB2; damage-specific DNA binding protein 2, 48kDa; damage specific DNA binding protein 2 (48kD); DNA damage-binding protein 2; DDB p48 subunit; DDBB; FLJ34321; UV damaged DNA binding protein 2; UV DDB2; xeroderma pigmentosum group E protein; UV-DDB 2; UV-damaged DNA-binding protein 2; damage-specific DNA-binding protein 2; UV-DDB2
• Gene ID: 1643
• mRNA Refseq: NM_000107
• Protein Refseq: NP_000098
• MIM: 600811
• UniProt ID: Q92466