Recombinant Human DDB2 293 Cell Lysate

• Cat.No.: DDB2-7029HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for damage-specific DNA binding protein 2, 48kDa (DDB2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: DDB2 damage-specific DNA binding protein 2, 48kDa [ Homo sapiens ]
• Official Symbol: DDB2
• Synonyms: DDB2; damage-specific DNA binding protein 2, 48kDa; damage specific DNA binding protein 2 (48kD); DNA damage-binding protein 2; DDB p48 subunit; DDBB; FLJ34321; UV damaged DNA binding protein 2; UV DDB2; xeroderma pigmentosum group E protein; UV-DDB 2; UV-damaged DNA-binding protein 2; damage-specific DNA-binding protein 2; UV-DDB2
• Gene ID: 1643
• mRNA Refseq: NM_000107
• Protein Refseq: NP_000098
• MIM: 600811
• UniProt ID: Q92466
• Chromosome Location: 11p12-p11
• Pathway: Cul4-DDB1-DDB2 complex, organism-specific biosystem; DNA Repair, organism-specific biosystem; Direct p53 effectors, organism-specific biosystem; Dual incision reaction in GG-NER, organism-specific biosystem; Formation of incision complex in GG-NER, organism-specific biosystem; Global Genomic NER (GG-NER), organism-specific biosystem; Nucleotide Excision Repair, organism-specific biosystem
• Function: DNA binding; damaged DNA binding; protein binding; contributes_to ubiquitin-protein ligase activity