Recombinant Human DDAH1, His-tagged

• Cat.No.: DDAH1-2846H
• Product Overview: Recombinant human DDAH1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 33.5 kDa (308aa) confirmed by MALDI-TOF.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Dimethylarginine dimethylaminohydrolase 1, also known DDAH1, belongs to the dimethylarginine dimethylaminohydrolase gene family. The DDAH1 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. Impairment of DDAH1 causes ADMA (asymmetric dimethylarginine) accumulation and a reduction in cGMP generation.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MGSMAGLGHP AAFGRATHAV VRALPESLGQ HALRSAKGEE VDVARAERQH QLYVGVLGSK LGLQVVELPA DESLPDCVFV EDVAVVCEET ALITRPGAPS RRKEVDMMKE ALEKLQLNIV EMKDENATLD GGDVLFTGRE FFVGLSKRTN QRGAEILADT FKDYAVSTVP VADGLHLKSF CSMAGPNLIA IGSSESAQKA LKIMQQMSDH RYDKLTVPDD IAANCIYLNI PNKGHVLLHR TPEEYPESAK VYEKLKDHML IPVSMSELEK VDGLLTCCSV LINKKVDS
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: DDAH1 dimethylarginine dimethylaminohydrolase 1 [ Homo sapiens ]
• Synonyms: DDAH1; dimethylarginine dimethylaminohydrolase 1; DDAH; FLJ21264; FLJ25539; N(G),N(G)-dimethylarginine dimethylaminohydrolase 1; DDAHI; DDAH-1; dimethylargininase-1; NG, NG-dimethylarginine dimethylaminohydrolase; EC 3.5.3.18
• Gene ID: 23576
• mRNA Refseq: NM_001134445
• Protein Refseq: NP_001127917
• MIM: 604743
• UniProt ID: O94760
• Chromosome Location: 1p22
• Function: catalytic activity; dimethylargininase activity; hydrolase activity; metal ion binding