Recombinant Human DCXR 293 Cell Lysate

• Cat.No.: DCXR-7030HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for dicarbonyl/L-xylulose reductase (DCXR) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: DCXR dicarbonyl/L-xylulose reductase [ Homo sapiens ]
• Official Symbol: DCXR
• Synonyms: DCXR; dicarbonyl/L-xylulose reductase; L-xylulose reductase; DCR; KIDCR; SDR20C1; short chain dehydrogenase/reductase family 20C; member 1; carbonyl reductase 2; carbonyl reductase II; sperm surface protein P34H; kidney dicarbonyl reductase; short chain dehydrogenase/reductase family 20C, member 1; XR; HCR2; P34H; HCRII
• Gene ID: 51181
• mRNA Refseq: NM_001195218
• Protein Refseq: NP_001182147
• MIM: 608347
• UniProt ID: Q7Z4W1
• Chromosome Location: 17q25.3
• Pathway: D-glucuronate degradation I, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Pentose and glucuronate interconversions, organism-specific biosystem; Pentose and glucuronate interconversions, conserved biosystem
• Function: L-xylulose reductase (NADP+) activity; nucleotide binding; oxidoreductase activity, acting on CH-OH group of donors; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor