Recombinant Human DCTN3, GST-tagged

• Cat.No.: DCTN3-11866H
• Product Overview: Recombinant Human DCTN3 protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Description: This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing of this gene generates 2 transcript variants.
• Source: E.coli
• Species: Human
• Tag: GST
• Protein length: 1-186a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: DCTN3 dynactin 3 (p22) [ Homo sapiens ]
• Official Symbol: DCTN3
• Synonyms: DCTN3; dynactin 3 (p22); dynactin subunit 3; DCTN 22; p22; dynactin light chain; dynactin 3, isoform 1; dynactin complex subunit 22 kDa subunit; DCTN22; DCTN-22; MGC111190
• Gene ID: 11258
• mRNA Refseq: NM_007234
• Protein Refseq: NP_009165
• MIM: 607387
• UniProt ID: O75935
• Chromosome Location: 9p13
• Pathway: Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Centrosome maturation, organism-specific biosystem; G2/M Transition, organism-specific biosystem; Loss of Nlp from mitotic centrosomes, organism-specific biosystem; Loss of proteins required for interphase microtubule organization??from the centrosome, organism-specific biosystem; Mitotic G2-G2/M phases, organism-specific biosystem
• Function: protein binding; structural molecule activity