Recombinant Human DCLK1 protein(Met1-Val705)

• Cat.No.: DCLK1-2365H
• Product Overview: Recombinant Human DCLK1 ( O15075-1) (Met 1-Val 705) was expressed and purified in Insect Cells with two additional amino acids (Gly & Pro ) at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: Non
• Protein length: Met1-Val705
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7. 4.
• Bio-activity: The specific activity was determined to be > 3.5 nmol/min/mg using synthetic Autocamtide-2 peptide (KKALRRQETVDAL-amide) as substrate.
• Molecular Mass: The secreted recombinant human DCLK1 consists of 707 amino acids and predicts a molecular mass of 78.5 KDa. The apparent molecular mass of the protein is approximately 64 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DCLK1 doublecortin-like kinase 1 [ Homo sapiens ]
• Official Symbol: DCLK1
• Synonyms: DCLK1; doublecortin-like kinase 1; DCAMKL1, doublecortin and CaM kinase like 1; serine/threonine-protein kinase DCLK1; DCDC3A; DCLK; KIAA0369; doublecortin and CaM kinase-like 1; doublecortin-like and CAM kinase-like 1; doublecortin domain-containing protein 3A; CL1; CLICK1; DCAMKL1
• Gene ID: 9201
• mRNA Refseq: NM_001195415
• Protein Refseq: NP_001182344
• MIM: 604742
• UniProt ID: O15075