Recombinant Human D-dopachrome Tautomerase, His-tagged

• Cat.No.: DDT-734H
• Product Overview: Recombinant human DDT, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW = 14.8 kDa(138aa).

Specification

• Cat. No.: DDT-734H
• Description: DDT is an enzyme that catayzes the tautomerization of D-dopachrome to give 5,6-dihydroxyindole (DHI). This protein belongs to the family oflyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33% identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity. In addition, it may functions a proinflammatory cytokine.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MPFLELDTNL PANRVPAGLE KRLCAAAASI LGKPADRVNV TVRPGLAMAL SGSTEPCAQL SISSIGVVGT AEDNRSHSAH FFEFLTKELA LGQDRILIRF FPLESWQIGK IGTVMTFL
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: DDT D-dopachrome tautomerase [ Homo sapiens ]
• Synonyms: DDT; D-dopachrome tautomerase; DDCT; D-dopachrome decarboxylase; OTTHUMP00000198529; phenylpyruvate tautomerase II; EC 4.1.1.84
• Gene ID: 1652
• mRNA Refseq: NM_001084392
• Protein Refseq: NP_001077861
• MIM: 602750
• UniProt ID: P30046
• Chromosome Location: 22q11.23
• Function: D-dopachrome decarboxylase activity; dopachrome isomerase activity; lyase activity; protein binding