Recombinant Human CYP7A1 293 Cell Lysate

• Cat.No.: CYP7A1-7099HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CYP7A1 cytochrome P450, family 7, subfamily A, polypeptide 1 [ Homo sapiens ]
• Official Symbol: CYP7A1
• Synonyms: CYP7A1; cytochrome P450, family 7, subfamily A, polypeptide 1; CYP7, cytochrome P450, subfamily VIIA (cholesterol 7 alpha monooxygenase), polypeptide 1; cholesterol 7-alpha-monooxygenase; cholesterol 7 alpha monooxygenase; cytochrome P450 7A1; cholesterol 7-alpha-hydroxylase; cytochrome P450, subfamily VIIA polypeptide 1; CP7A; CYP7; CYPVII; MGC126826; MGC138389
• Gene ID: 1581
• mRNA Refseq: NM_000780
• Protein Refseq: NP_000771
• MIM: 118455
• UniProt ID: P22680
• Chromosome Location: 8q11-q12
• Pathway: Bile acid and bile salt metabolism, organism-specific biosystem; Bile acid biosynthesis, cholesterol =>cholate, organism-specific biosystem; Bile acid biosynthesis, cholesterol => cholate, conserved biosystem; Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem
• Function: cholesterol 7-alpha-monooxygenase activity; electron carrier activity; heme binding; metal ion binding; monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen