Recombinant Human CYP46A1, His-tagged

• Cat.No.: CYP46A1-11786H
• Product Overview: Recombinant Human CYP46A1 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: C-term-300a.a.
• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: CYP46A1 cytochrome P450, family 46, subfamily A, polypeptide 1 [ Homo sapiens ]
• Official Symbol: CYP46A1
• Synonyms: CYP46A1; cytochrome P450, family 46, subfamily A, polypeptide 1; CYP46, cytochrome P450, subfamily 46 (cholesterol 24 hydroxylase); cholesterol 24-hydroxylase; CH24H; cytochrome P450 46A1; cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase); CP46; CYP46
• Gene ID: 10858
• mRNA Refseq: NM_006668
• Protein Refseq: NP_006659
• MIM: 604087
• UniProt ID: Q9Y6A2
• Chromosome Location: 14q32.1
• Pathway: Bile acid and bile salt metabolism, organism-specific biosystem; Biological oxidations, organism-specific biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Endogenous sterols, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Phase 1 - Functionalization of compounds, organism-specific biosystem
• Function: cholesterol 24-hydroxylase activity; electron carrier activity; heme binding; metal ion binding; monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; steroid hydroxylase activity