Recombinant Human CYP3A5, GST-tagged

• Cat.No.: CYP3A5-11785H
• Product Overview: Recombinant Human CYP3A5 protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Description: This gene,CYP3A5, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. The enzyme metabolizes drugs such as nifedipine and cyclosporine as well as the steroid hormones testosterone, progesterone and androstenedione. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. This cluster includes a pseudogene, CYP3A5P1, which is very similar to CYP3A5. This similarity has caused some difficulty in determining whether cloned sequences represent the gene or the pseudogene. Multiple alternatively spliced transcript variants have been identified for this gene.
• Source: E.coli
• Species: Human
• Tag: GST
• Protein length: 152-502a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: CYP3A5 cytochrome P450, family 3, subfamily A, polypeptide 5 [ Homo sapiens ]
• Official Symbol: CYP3A5
• Synonyms: CYP3A5; cytochrome P450, family 3, subfamily A, polypeptide 5; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 5; cytochrome P450 3A5; CP35; P450PCN3; PCN3; cytochrome P-450; niphedipine oxidase; cytochrome P450 HLp2; cytochrome P450-PCN3; microsomal monooxygenase; xenobiotic monooxygenase; aryl hydrocarbon hydroxylase; flavoprotein-linked monooxygenase; CYPIIIA5; FLJ31317; DKFZp686L16231
• Gene ID: 1577
• mRNA Refseq: NM_000777
• Protein Refseq: NP_000768
• MIM: 605325
• UniProt ID: P20815
• Chromosome Location: 7q21.1
• Pathway: Biological oxidations, organism-specific biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Drug metabolism - cytochrome P450, organism-specific biosystem; Drug metabolism - cytochrome P450, conserved biosystem; Drug metabolism - other enzymes, organism-specific biosystem; Drug metabolism - other enzymes, conserved biosystem; Irinotecan Pathway, organism-specific biosystem
• Function: aromatase activity; electron carrier activity; heme binding; metal ion binding; monooxygenase activity; oxidoreductase activity; oxygen binding