Recombinant Human CYP2E1 protein, His & GST-tagged

Cat.No. : CYP2E1-5702H
Product Overview : Recombinant Human CYP2E1 aa. (Pro36~Val271) fused with N-terminal His & GST tag was produced in E. coli cells.
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Species : Human
Source : E.coli
Tag : GST&His
Protein Length : Pro36~Val271
Description : This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.
Form : Freeze-dried powder
Molecular Mass : 68kDa as determined by SDS-PAGE reducing conditions.
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 9.3.
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200μg/mL
Storage buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name CYP2E1 cytochrome P450 family 2 subfamily E member 1 [ Homo sapiens (human) ]
Official Symbol CYP2E1
Synonyms CYP2E1; cytochrome P450 family 2 subfamily E member 1; CPE1; CYP2E; P450-J; P450C2E; cytochrome P450 2E1; 4-nitrophenol 2-hydroxylase; CYPIIE1; cytochrome P450, family 2, subfamily E, polypeptide 1; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1; cytochrome P450-J; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase
Gene ID 1571
mRNA Refseq NM_000773.3
Protein Refseq NP_000764.1
UniProt ID P05181

Not For Human Consumption!

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