Recombinant Human CYP2C19/NADPH protein, Active

• Cat.No.: CYP2C19-362H
• Product Overview: This is a permeabilized and stabilized dried yeast powder preparation containing recombinant human CYP2C19 and recombinant human P450 NADPH oxidoreductase co expressed in the same preparation.

Specification

• Species: Human
• Source: P.pastoris
• Tag: Non
• Form: Lyophilized powder
• Molecular Mass: 55 Kda
• Notes: Centrifuge the vial prior to opening
• Storage: Stable for 1 year as supplied. Store dry powder at -20 centigrade. Reconstitute in 50 mM Potassium Phosphate buffer, pH 7.7. Not recommended to be stored as solution. If storing as solution, aliquot and store at -80 centigrade. Avoid repeated freeze/thaw cycles and use aliquots within one month (the human CYP2C19 will lose approximately 10% activity per week when stored at -80 centigrade). Thaw aliquots rapidly at 37 centigrade and place on ice until use (thawed aliquots should be used within 4 hours).
• Reconstitution: 50 mM Potassium Phosphate buffer, pH 7.7

Gene Information

• Gene Name: CYP2C19 cytochrome P450, family 2, subfamily C, polypeptide 19 [ Homo sapiens ]
• Official Symbol: CYP2C19
• Synonyms: CYP2C19; cytochrome P450, family 2, subfamily C, polypeptide 19; CYP2C, cytochrome P450, subfamily IIC (mephenytoin 4 hydroxylase), polypeptide 19; cytochrome P450 2C19; CPCJ; P450IIC19; CYPIIC17; CYPIIC19; cytochrome P450-11A; cytochrome P450-254C; cytochrome P-450 II C; microsomal monooxygenase; xenobiotic monooxygenase; mephenytoin 4-hydroxylase; S-mephenytoin 4-hydroxylase; (R)-limonene 6-monooxygenase; (S)-limonene 6-monooxygenase; (S)-limonene 7-monooxygenase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase), polypeptide 19; CYP2C; P450C2C
• Gene ID: 1557
• mRNA Refseq: NM_000769
• Protein Refseq: NP_000760
• MIM: 124020
• UniProt ID: P33261
• Chromosome Location: 10q24
• Pathway: Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Biological oxidations, organism-specific biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Drug metabolism - cytochrome P450, organism-specific biosystem; Drug metabolism - cytochrome P450, conserved biosystem; Linoleic acid metabolism, organism-specific biosystem
• Function: (R)-limonene 6-monooxygenase activity; (S)-limonene 6-monooxygenase activity; (S)-limonene 7-monooxygenase activity; electron carrier activity; enzyme binding; heme binding; metal ion binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; oxygen binding; steroid hydroxylase activity