Recombinant Human/Cynomolgus/Rhesus CD28 protein(Met1-Pro152), His&Avi-tagged, Biotinylated

• Cat.No.: CD28-1056H
• Product Overview: Biotinylated Recombinant Human/Cynomolgus/Rhesus CD28 (P10747-1) (Met1-Pro152) was expressed in HEK293 with a C-terminal polyhistidine tag followed by an AVI tag.

Specification

• Source: HEK293
• Species: Human/Cynomolgus/Rhesus
• Tag: C-His&Avi
• Protein length: Met1-Pro152
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Recombinant Mouse CD80 / B7-1 Protein (Fc Tag) at 2 μg/ml (100 μl/well) can bind Recombinant Human/Cynomolgus/Rhesus CD28 Protein(ECD,His&AVI Tag),Biotinylated, The EC50 is 800-2100 ng/mL.
• Molecular Mass: The recombinant Human/Cynomolgus/Rhesus CD28 consists of 160 amino acids and predicts a molecular mass of 18.40 kDa. It migrates as an approximately 41.73 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: CD28