Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein(Asn 19 - Pro 152)

• Cat.No.: CD28-4995H
• Product Overview: Recombinant Human / Cynomolgus / Rhesus macaque CD28 protein(Asn 19 - Pro 152)(P10747-1), fused with N-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human / Cynomolgus / Rhesus macaque
• Source: HEK293
• Tag: Fc
• Protein Length: Asn 19 - Pro 152
• Form: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human/Cynomolgus/Rhesus macaque CD28, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD28 Antibody, hFc Tag with the EC50 of 46.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per ug by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -80°C for 3-6 months after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CD28 CD28 molecule [ Homo sapiens ]
• Official Symbol: CD28
• Synonyms: CD28; CD28 molecule; CD28 antigen (Tp44); T-cell-specific surface glycoprotein CD28; T cell specific surface glycoprotein; CD28 antigen; Tp44; MGC138290
• Gene ID: 940
• mRNA Refseq: NM_001243077
• Protein Refseq: NP_001230006
• MIM: 186760
• UniProt ID: P10747