Recombinant Human Cyclin-Dependent Kinase 4, GST-tagged

• Cat.No.: CDK4-1000H
• Product Overview: Recombinant full-length human CDK4 was expressed in E. coli cells using a N-terminal GST tag.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Description: CDK4 is a member of the cyclin-dependent protein kinases and is involved in the control of cell proliferation during the G1 phase of the cell cycle. CDK4 forms a complex with the D-type cyclins which regulate its activity. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 can mediate phosphorylation of the C-terminal region of RB protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. Pharmacologic inactivation of CDC37/HSP90 function leads to reduced stability of CDK4. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 57 kDa
• Expression System: E. coli
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
• Purity: > 80 %
• Concentration: 0.2 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: ATF-2 transcription factor network; B Cell Receptor Signaling Pathway; Bladder cancer; Calcineurin-regulated NFAT-dependent transcription in lymphocytes; Cell Cycle; Cell cycle; Chronic myeloid leukemia; Cyclin D associated events in G1; Diabetes pathways; FOXM1 transcription factor network; G1 Phase; G1 to S cell cycle control; Glioma; HTLV-I infection; IL-7 Signaling Pathway

Gene Information

• Gene Name: CDK4 cyclin-dependent kinase 4 [ Homo sapiens ]
• Official Symbol: CDK4
• Synonyms: CDK4; cyclin-dependent kinase 4; CMM3; PSK-J3; MGC14458; Cyclin-dependent kinase 4; EC 2.7.11.22; Cell division protein kinase 4; cell division kinase 4; melanoma cutaneous malignant, 3
• Gene ID: 1019
• mRNA Refseq: NM_000075
• Protein Refseq: NP_000066
• MIM: 123829
• UniProt ID: P11802
• Chromosome Location: 12q14
• Function: ATP binding; cyclin-dependent protein kinase activity; nucleotide binding; protein binding; protein kinase activity