Recombinant Human CXCR2 HEK293T cell lysate

• Cat.No.: CXCR2-22HCL
• Product Overview: Human CXCR2, transcript variant 1, over-expression lysate was derived from Human HEK293T cell line.

Specification

• Source: Human HEK293T cell line
• Species: Human
• Tag: Myc&DDK
• Form: The cell lysate is provided in RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4).
• Molecular Mass: 40.6 kDa
• Usage: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
  1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
• Storage: The lysate is shipped with dry ice. Upon receiving, store the sample at -20centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20centigrade. Avoid repeated freeze-thaw cycles.
  Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: CXCR2 chemokine (C-X-C motif) receptor 2 [ Homo sapiens ]
• Official Symbol: CXCR2
• Synonyms: CXCR2; chemokine (C-X-C motif) receptor 2; IL8RB, interleukin 8 receptor, beta; C-X-C chemokine receptor type 2; CD182; CMKAR2; CXC-R2; CXCR-2; IL-8R B; GRO/MGSA receptor; IL-8 receptor type 2; interleukin 8 receptor B; chemokine (CXC) receptor 2; interleukin 8 receptor, beta; interleukin 8 receptor type 2; interleukin-8 receptor type B; CXCR2 gene for IL8 receptor type B; high affinity interleukin-8 receptor B; IL8R2; IL8RA; IL8RB; CDw128b
• Gene ID: 3579
• mRNA Refseq: NM_001168298
• Protein Refseq: NP_001161770
• MIM: 146928
• UniProt ID: P25025
• Chromosome Location: 2q35
• Pathway: Chemokine receptors bind chemokines, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Endocytosis, organism-specific biosystem
• Function: C-X-C chemokine receptor activity; G-protein coupled receptor activity; interleukin-8 binding; interleukin-8 receptor activity; receptor activity; signal transducer activity