Recombinant Human CTSE, His-tagged

• Cat.No.: CTSE-5090H
• Product Overview: Recombinant human CTSE protein, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: CTSE is a gastric aspartyl protease that functions as a disulfide-linked homodimer. CTSE, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen. Transcript variants utilizing alternative polyadenylation signals and two transcript variants encoding different isoforms exist for this gene.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 35.4 kDa (330aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSTESCSMD QSAKEPLINY LDMEYFGTIS IGSPPQNFTV IFDTGSSNLW VPSVYCTSPA CKTHSRFQPS QSSTYSQPGQ SFSIQYGTGS LSGIIGADQV SVEGLTVVGQ QFGESVTEPG QTFVDAEFDG ILGLGYPSLA VGGVTPVFDN MMAQNLVDLP MFSVYMSSNP EGGAGSELIF GGYDHSHFSG SLNWVPVTKQ AYWQIALDNM LWSVPTLTSC RMSPSPLTES PIPSAQLPTP YWTSWMECSS AAVAFKDLTS TLQLGPSGSW GMSSFDSFTQ SLTVGITVWD WPQQSPKEGP CVCACLSDRP
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CTSE cathepsin E [ Homo sapiens ]
• Official Symbol: CTSE
• Synonyms: CTSE; cathepsin E; slow-moving proteinase; erythrocyte membrane aspartic proteinase; CATE
• Gene ID: 1510
• mRNA Refseq: NM_001910
• Protein Refseq: NP_001901
• MIM: 116890
• UniProt ID: P14091
• Chromosome Location: 1q32.1
• Pathway: Lysosome, organism-specific biosystem; Lysosome, conserved biosystem
• Function: aspartic-type endopeptidase activity; peptidase activity