Recombinant Human CTSD, His-tagged

• Cat.No.: CTSD-218H
• Product Overview: Recombinant Human CTSD MS Standard Protein, C13 and N15-labeled (CTSD / Cathepsin D, Heavy Labeled) Ser 19 - Leu 412 (Accession # NP_001900) was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain >99% incorpor

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 19-412 a.a.
• Description: Cathepsin D is also known as CTSD, CPSD, which belongs to the peptidase A1 family. Cathepsin D can be cleaved into the following 2 chains: cathepsin D light chain and cathepsin D heavy chain, which is expressed in the aorta extrcellular space (at protein level). The catalytic activity of Cathepsin D is specificity similar to, but narrower than, that of pepsin A. Cathepsin D does not cleave the 4-Gln-|-His-5 bond in B chain of insulin. Cathepsin D involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Molecular Mass: CTSD / Cathepsin D, Heavy Labeled is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 43.6 kDa. The predicted N-terminus is Ser 19. DTT-reduced Protein migrates as 45-55 kDa in SDS-PAGE due to glycosylation.CTSD / Cathepsin D,
• Endotoxin: Less than 1.0 EU per μg of the CTSD / Cathepsin D, Heavy Labeled by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
• Reconstitution: See Certificate of Analysis for reconstitution instructions and specific concentrations.

Gene Information

• Gene Name: CTSD cathepsin D [ Homo sapiens ]
• Official Symbol: CTSD
• Synonyms: CTSD; cathepsin D; cathepsin D (lysosomal aspartyl protease) , CPSD; ceroid lipofuscinosis; neuronal 10; CLN10; lysosomal aspartyl protease; lysosomal aspartyl peptidase; ceroid-lipofuscinosis, neuronal 10; CPSD; MGC2311
• Gene ID: 1509
• mRNA Refseq: NM_001909
• Protein Refseq: NP_001900
• MIM: 116840
• UniProt ID: P07339
• Chromosome Location: 11p15.5
• Pathway: Ceramide signaling pathway, organism-specific biosystem; Direct p53 effectors, organism-specific biosystem; LKB1 signaling events, organism-specific biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Tuberculosis, organism-specific biosystem; Tuberculosis, conserved biosystem
• Function: aspartic-type endopeptidase activity; peptidase activity