Recombinant Human CTNNB1 protein(Met1-Leu781), His&GST-tagged

• Cat.No.: CTNNB1-3891H
• Product Overview: Recombinant Human CTNNB1 (P35222-1) (Met 1-Leu 781) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-781 a.a.
• Form: Lyophilized from sterile 50mM Tris, 150mM NaCl, 25% glycerol, pH 8.0, 0.1mM EDTA, 1mM TCEP, 0.4mM PMSF, 0.5mM GSH Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human CTNNB1/GST chimera consists of 1018 amino acids and has a calculated molecular mass of 113 kDa. It migrates as an approximately 116 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CTNNB1 catenin (cadherin-associated protein), beta 1, 88kDa [ Homo sapiens ]
• Official Symbol: CTNNB1
• Synonyms: CTNNB1; catenin (cadherin-associated protein), beta 1, 88kDa; catenin (cadherin associated protein), beta 1 (88kD) , CTNNB; catenin beta-1; beta catenin; CTNNB; FLJ25606; FLJ37923; DKFZp686D02253
• Gene ID: 1499
• mRNA Refseq: NM_001098209
• Protein Refseq: NP_001091679
• MIM: 116806
• UniProt ID: P35222