Recombinant Human CST5, His-tagged

• Cat.No.: CST5-102H
• Product Overview: Recombinant human CST5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: CST5, also known as Cystatin-D, is a novel member of the cystatin superfamily. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. Two alleles of the cystatin D gene (CST5), encoding protein variants with either Cys or Arg as residue 26 in their 122-residue polypeptide chains, are present in the population.
• Form: Liquid. In phosphate buffered saline (pH7.4) containing 10% glycerol.
• Molecular Mass: 14.36kDa (123aa)(13.5-18kDa in SDS-PAGE under reducing conditions.)
• AA Sequence: QSRTLAGGIH ATDLNDKSVQ RALDFAISEY NKVINKDEYY SRPLQVMAAY QQIVGGVNYY FNVKFGRTTC TKSQPNLDNC PFNDQPKLKEEEFCSFQINE VPWEDKISIL NYKCRKVHHH HHH
• Endotoxin: < 1.0 eu per 1 microgram of protein (determined by lal
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by absorbance at 280nm)

Gene Information

• Gene Name: CST5 cystatin D [ Homo sapiens ]
• Official Symbol: CST5
• Synonyms: CST5; cystatin D; cystatin-D; cystatin 5; cystatin-5; cysteine-proteinase inhibitor; MGC71922
• Gene ID: 1473
• mRNA Refseq: NM_001900
• Protein Refseq: NP_001891
• MIM: 123858
• UniProt ID: P28325
• Chromosome Location: 20p11.21
• Pathway: Endochondral Ossification, organism-specific biosystem; Salivary secretion, organism-specific biosystem; Salivary secretion, conserved biosystem
• Function: cysteine-type endopeptidase inhibitor activity; peptidase inhibitor activity; protein binding