Recombinant Human CSRP1 protein(Met1-Glu193), His-tagged

• Cat.No.: CSRP1-3085H
• Product Overview: Recombinant Human CSRP1 (NP_004069.1) (Met 1-Glu 193) was expressed in E. coli, with a polyhistide tag at the C-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Met1-Glu193
• Form: Lyophilized from sterile 10mM Na2HPO4, 2mM KH2PO4, 2.7mM KCl, 500mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human CSRP1 consisting of 199 amino acids and has a calculated molecular mass of 21.4 kDa. It migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CSRP1 cysteine and glycine-rich protein 1 [ Homo sapiens ]
• Official Symbol: CSRP1
• Synonyms: CSRP1; cysteine and glycine-rich protein 1; CYRP; CSRP; D1S181E; LIM-domain protein; cysteine-rich protein 1; CRP; CRP1; DKFZp686M148
• Gene ID: 1465
• mRNA Refseq: NM_001193570
• Protein Refseq: NP_001180499
• MIM: 123876
• UniProt ID: P21291

Case Study

Case 1: Järvinen PM, et al. J Cell Physiol. 2012

Transforming growth factor-β (TGF-β) is a key player in a bunch of body processes like cell growth and differentiation, but when it goes haywire, it’s linked to fibrosis and other issues. Researchers found cysteine-rich protein 1 (CRP1) shoots up when TGF-β1 enters the scene, with a quick spike followed by a longer-lasting rise, thanks to Smad and p38 MAPK pathways. CRP1 is not just hanging around; it’s driving myofibroblast changes and boosting cell contractility. In lungs affected by idiopathic pulmonary fibrosis (IPF), CRP1 is prominently found in fibrous areas, way more than in healthy tissue, making it a potential target for understanding and tackling IPF.

Fig1. Western analysis of CRP1 expression in A549 cells treated with TGF-β1 (5 ng/ml) for the indicated times.

Fig2. Western analysis of CRP1 in A549 cells treated with TGF-β1 (5 ng/ml) for 15, 30, 45, or 60 min.

Case 2: Margets A, et al. Mol Plant Microbe Interact. 2024

The soybean cyst nematode (SCN) infiltrates plants by releasing proteins, including a crafty cysteine protease named CPR1, which disrupts plant defenses. CPR1 is consistent across all SCN strains, indicating its crucial role. It targets soybeans' GmBCAT1 enzyme, making it easier for the nematode to thrive. When researchers lower CPR1 levels, nematode invasion drops; when CPR1 is present in soybeans, they get more vulnerable. This makes CPR1 a key target for developing plant protection strategies.

Fig1. Immunoblot showing expression of the RPS5D266E and CPR1C323S or CPR1.

Fig2. GmBCAT1∆1-74 accumulation is reduced when co-expressed with active CPR1 variants.

Application

Recombinant CSRP1 protein plays a fascinating role in biomedical research, particularly when it comes to understanding how cells stick together and communicate. This protein is all about structure—giving cells what they need to grab onto one another, which is crucial when studying muscle development and repair. For scientists digging into muscle disorders or cardiac conditions, CSRP1 provides valuable insights into how cells manage to form those all-important connections that keep our muscles functioning smoothly. Moreover, CSRP1 isn’t just a player in the muscle world; it's also eyed for its role in cancer research. Researchers are keen on deciphering its involvement in how cancer cells might move and invade other tissues. By understanding this protein's behavior better, the hope is to develop new ways to stall or even prevent the spread of cancers. It’s like piecing together a puzzle that could eventually lead to therapies capable of halting metastasis, making it a significant topic of interest for many in the oncology field.