Recombinant Human CSPG4 protein, His-tagged

• Cat.No.: CSPG4-1533H
• Product Overview: Recombinant Human CSPG4 aa. (Ser1705~Ile1942) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ser1705~Ile1942
• Description: A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
• Form: Freeze-dried powder
• Molecular Mass: 29kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: >98%
• Characteristic: The isoelectric point is 6.4.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive
  Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200µg/mL
• Storage buffer: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: CSPG4 chondroitin sulfate proteoglycan 4 [ Homo sapiens (human) ]
• Official Symbol: CSPG4
• Synonyms: NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan NG2; melanoma chondroitin sulfate proteoglycan; melanoma-associated chondroitin sulfate proteoglycan; chondroitin sulfate proteoglycan 4
• Gene ID: 1464
• mRNA Refseq: NM_001897.4
• Protein Refseq: NP_001888.2
• UniProt ID: Q6UVK1