Recombinant Human CSF3R protein(Met1-Pro621), hFc-tagged

• Cat.No.: CSF3R-164H
• Product Overview: Recombinant Human G-CSF receptor (NP_000751.1)(Met 1-Pro 621) was expressed in HEK293 with the fused Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-621 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit GCSF-induced proliferation of NFS60 mouse myeloid cells. The ED50 for this ettect is typically 2-20 ng/ml in the presence of 0.125ng/ml of recombinant human GCSF.
• Molecular Mass: The mature recombinant human G-CSFR/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 835 amino acids and predicts a molecular mass of 93.3 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rh GCSFR/Fc monomer is approximately 120-130 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CSF3R colony stimulating factor 3 receptor (granulocyte) [ Homo sapiens ]
• Official Symbol: CSF3R
• Synonyms: CSF3R; colony stimulating factor 3 receptor (granulocyte); CD114; granulocyte colony-stimulating factor receptor; GCSFR; G-CSF-R; CD114 antigen; G-CSF receptor
• Gene ID: 1441
• mRNA Refseq: NM_000760
• Protein Refseq: NP_000751
• MIM: 138971
• UniProt ID: Q99062

Case Study

Case 1: Zhang H, et al. J Biol Chem. 2018

Granulocyte colony-stimulating factor (G-CSF) and its receptor CSF3R play crucial roles in blood cell production. Mutations in CSF3R can cause blood cancers. In this study, researchers found new mutations that promote cancer by changing how the receptor functions. These changes affect its interaction and signaling, helping us understand how certain genetic changes can lead to cancer.

Fig1. CSF3R expression determined by nonreducing (upper panel) and reducing (lower panel) immunoblots.

Fig2. Surface expression of CSF3R of Ba/F3 cells expressing CSF3R WT, E524K, and E524K/L776A/L777A was determined by FACS.

Case 2: Rohrabaugh S, et al. Leukemia. 2017

CSF3R mutations linked to chronic neutrophilic leukemia (CNL) involve both membrane-proximal and truncation changes. Alone, truncation can’t cause leukemia, but when combined with proximal mutations, they do, with varying onset times. This study found that heightened Mapk signaling is crucial in this, and inhibiting MEK1/2 with trametinib effectively suppresses the leukemia, even when resistant to other treatments. This positions MEK1/2 inhibition as a promising treatment strategy for CNL.

Fig1. Immunoblots from the total cell extracts of BaF3 cells expressing CSF3R variants probed with anti-HA to determine the expression CSF3R.

Fig2. Graphs showing the total WBC levels (top panel) and GFP positive cells as a surrogate leukemic burden (bottom panel) from the peripheral blood of mice transplanted with CSF3R-T618I.

Application

Recombinant CSF3R protein, also known as the granulocyte colony-stimulating factor receptor (G-CSFR), is pivotal in various therapeutic and research applications. It acts as a key part of the signaling pathway for granulocyte production and can be invaluable in treating conditions where enhanced neutrophil production is beneficial, such as in specific chemotherapy-induced neutropenia. Essentially, its recombinant form helps in simulating these bodily functions for medical purposes. Moreover, these recombinant proteins are often used in a lab setting for research or to generate antibodies. Scientists might employ these proteins to investigate cell signaling pathways further or in drug development to test new treatments aimed at modulating immune system responses. While it’s a specialized field, the technology that supports the creation and application of recombinant proteins like CSF3R is fundamental to advancing modern medicine and understanding diseases at a molecular level.

Fig1. Inhibitors of signaling pathways downstream of G-CSFR. (Clifford Liongue, 2014)