Recombinant Human CSE1L Over-expression Cell Lysate

Cat.No. : CSE1L-9523HCL
  • Specification
  • Gene Information
  • Related Products
  • Download
Species : Human
Source : HEK293
Tag : DDK&Myc
Description : Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, the encoded protein may play a role both in apoptosis and in cell proliferation. Alternatively spliced transcript variants have been found for this gene.
Form : 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4
Molecular Mass : 110.2 kDa
Usage : 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
Storage : Upon receiving, store the sample at -20 centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles.
Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name CSE1L CSE1 chromosome segregation 1-like (yeast) [ Homo sapiens ]
Official Symbol CSE1L
Synonyms CSE1L; CSE1 chromosome segregation 1-like (yeast); chromosome segregation 1 (yeast homolog) like; exportin-2; CAS; CSE1; XPO2; exp2; importin-alpha re-exporter; chromosome segregation 1-like protein; cellular apoptosis susceptibility protein; MGC117283; MGC130036; MGC130037;
Gene ID 1434
mRNA Refseq NM_001316
Protein Refseq NP_001307
MIM 601342
UniProt ID P55060
Chromosome Location 20q13
Pathway Direct p53 effectors, organism-specific biosystem; p53 pathway, organism-specific biosystem;
Function importin-alpha export receptor activity; protein binding; protein transporter activity;

Not For Human Consumption!

Inquiry

  • Reviews
  • Q&As

Customer Reviews (0)

Write a review

Q&As (0)

Ask a question

Ask a Question for All CSE1L Products

Required fields are marked with *

My Review for All CSE1L Products

Required fields are marked with *

0

Inquiry Basket

cartIcon