Recombinant Human Crystallin, Gamma C, His-tagged

• Cat.No.: CRYGC-2396H
• Product Overview: Recombinant humanCRYGC protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Gamma-crystallin C, alsoknown as CRYGC, belongs to the beta/gamma-crystallin family. Mammalian lenscrystallins are divided into alpha, beta, and gamma families; beta and gammacrystallins are also considered as a superfamily. Gamma-crystallins are ahomogeneous group of highly symmetrical, monomeric proteins typically lackingconnecting peptides and terminal extensions. They are differentiallyregulated after early development. Four gamma-crystallin genes (gamma-Athrough gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) areorganized in a genomic segment as a gene cluster. Whether due to aging ormutations in specific genes, gamma-crystallins have been involved in cataractformation. Defects in CRYGC are a cause of cataract autosomal dominant (ADC)and cataract Coppock-like (CCL)
• Form: Liquid. In 20 mMTris-HCl buffer, pH8.0, 10% glycerol, 2mM DTT, 200mM NaCl
• Molecular Weight: 23.5kDa (198aa),confirmed by MALDI-TOF
• Purity: > 95% by SDS -PAGE
• Concentration: 1mg/ml (determinedby Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMGKITF YEDRAFQGRS YETTTDCPNL QPYFSRCNSI RVESGCWMLY ERPNYQGQQYLLRRGEYPDY QQWMGLSDSI RSCCLIPQTV SHRLRLYERE DHKGLMMELS EDCPSIQDRF HLSEIRSLHVLEGCWVLYEL PNYRGRQYLL RPQEYRRCQD WGAMDAKAGS LRRVVDLY
• Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene name: CRYGCcrystallin, gamma C [ Homo sapiens ]
• Official Symbol: CRYGC
• Synonyms: CRYGC; crystallin, gamma C; CRYG3; gamma-crystallin C; CATARACT,VARIABLE ZONULAR PULVERULENT; CCL; CRGC_HUMAN; CRYG 3; Cryg 5; Cryg; CRYG3;CRYGC; Crystallin gamma 3; Crystallin gamma C; Gamma C crystallin; Gammacrystallin 2 1; Gamma crystallin 3; Gamma crystallin C; Gamma-C-crystallin;Gamma-crystallin 2-1; Gamma-crystallin 3; Gamma-crystallin C; Gammab cry;Len; OTTHUMP00000163869; gamma-C-crystallin; gamma-crystallin 3; crystallin,gamma-3; gamma-crystallin 2-1; CCL
• Gene ID: 1420
• mRNA Refseq: NM_020989
• Protein Refseq: NP_066269
• Uniprot ID: P07315
• Chromosome Location: 2q33-q35
• Function: protein binding; structural constituent of eye lens