Recombinant Human Crystallin Alpha A

• Cat.No.: CRYAA-309H
• Product Overview: Recombinant Human CRYA1 protein expressed in E.coli contains 173 (1-173) amino acids and has a molecular mass of 19.9 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 1-173 a.a.
• Description: Alpha crystallins are composed of two gene products; alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein(sHSP also known as the HSP20). They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function ofa-crystallins are an autokinase activity and participation in the intracellular architecture. The expression of alpha-A is preferentially restricted to the lens cell.
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 50 mM NaCl, 1 mM EDTA.
• Molecular Weight: 19.9 kDa (173aa).
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequence: MDVTIQHPWF KRTLGPFYPS RLFDQFFGEG LFEYDLLPFL SSTISPYYRQ SLFRTVLDSG ISEVRSDRDK FVIFLDVKHF SPEDLTVKVQ DDFVEIHGKH NERQDDHGYI SREFHRRYRL PSNVDQSALS CSLSADGMLT FCGPKIQTGL DATHAERAIP VSREEKPTSA PSS
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CRYAA
• Synonyms: CRYAA; crystallin, alpha A; CRYA1; HSPB4; HspB4; Heat shock protein beta-4; crystallin, alpha A; crystallin, alpha-1; human alphaA-crystallin (CRYA1).
• Gene ID: 1409
• mRNA Refseq: NM_000394.2
• Protein Refseq: NP_000385.1
• MIM: 123580
• UniProt ID: P02489
• Chromosome Location: 21q22.3
• Function: structural constituent of eye lens; unfolded protein binding;unfolded protein binding.