Recombinant Human CRK Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CRK-6643H
• Product Overview: CRK MS Standard C13 and N15-labeled recombinant protein (NP_058431) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 33.8 kDa
• AA Sequence: MAGNFDSEERSSWYWGRLSRQEAVALLQGQRHGVFLVRDSSTSPGDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPALLEFYKIHYLDTTTLIEPVSRSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDILRIRDKPEEQWWNAEDSEGKRGMIPVPYVEKYRPASASVSALIGGNQEGSHPQPLGGPEPGPYAQPSVNTPLPNLQNGPIYARVIQKRVPNAYDKTALALEVGELVKVTKINVSGQWEGECNGKRGHFPFTHVRLLDQQNPDEDFSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CRK CRK proto-oncogene, adaptor protein [ Homo sapiens (human) ]
• Official Symbol: CRK
• Synonyms: CRK; v-crk sarcoma virus CT10 oncogene homolog (avian); v crk avian sarcoma virus CT10 oncogene homolog; adapter molecule crk; proto-oncogene c-Crk; p38; CRKII; FLJ38130
• Gene ID: 1398
• mRNA Refseq: NM_016823
• Protein Refseq: NP_058431
• MIM: 164762
• UniProt ID: P46108