Recombinant Human CRK, His-tagged

• Cat.No.: CRK-2854H
• Product Overview: Recombinant human CRK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. MW = 25.0 kDa (224aa), confirmed by MALDI-TOF.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: CRK is a member of signaling adapter protein family that binds to several tyrosine-phosphorylated proteins. This protein is involved in many cellular processes including apoptosis, proliferation, and differentiation. It has a modular domain architecture consisting of an SH2 followed by two SH3 domains (src-homology domains). The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAGNFDSEER SSWYWGRLSR QEAVALLQGQ RHGVFLVRDS STSPGDYVLS VSENSRVSHY IINSSGPRPP VPPSPAQPPP GVSPSRLRIG DQEFDSLPAL LEFYKIHYLD TTTLIEPVSR SRQGSGVILR QEEAEYVRAL FDFNGNDEED LPFKKGDILR IRDKPEEQWW NAEDSEGKRG MIPVPYVEKY RPASASVSAL IGGR
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CRK v-crk sarcoma virus CT10 oncogene homolog (avian) [ Homo sapiens ]
• Synonyms: CRK; v-crk sarcoma virus CT10 oncogene homolog (avian); CRKII; adapter molecule crk; p38; proto-oncogene c-Crk; v-crk avian sarcoma virus CT10 oncogene homolog; avian sarcoma virus CT10 (v-crk) oncogene homolog
• Gene ID: 1398
• mRNA Refseq: NM_005206
• Protein Refseq: NP_005197
• MIM: 164762
• UniProt ID: P46108
• Chromosome Location: 17p13.3
• Pathway: Bacterial invasion of epithelial cells; Chemokine signaling pathway; Chronic myeloid leukemia; ErbB signaling pathway; Fc gamma R-mediated phagocytosis; Focal adhesion; Insulin signaling pathway; MAPK signaling pathway; Neurotrophin signaling pathway; Pathways in cancer; Regulation of actin cytoskeleton; Renal cell carcinoma; Shigellosis
• Function: SH2 domain binding; SH3/SH2 adaptor activity; protein binding; protein phosphorylated amino acid binding