Recombinant Human CREBBP protein, His&FLAG-tagged

• Cat.No.: CREBBP-2815H
• Product Overview: Recombinant Human CREBBP protein(accession number NM_004380.2)(1081-1197 aa) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His&C-FLAG
• Protein length: 1081-1197 aa
• Form: Supplied in 25 mM Tris pH 8.0, 150 mM NaCl, 10% glycerol.
• Bio-activity: 3 μM H4K5/8/12/16 (ac4) peptide was incubated with different concentrations of CREBBP (1081-1197) protein in 10 μl reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 μl FLAG antibody and SA-XL665 mixture (1:100 dilution in reaction buffer) was added to each reaction system and incubated for 1 hour. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
• Molecular Mass: 18.7 kDa
• Purity: >85% pure by SDS-PAGE.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CREBBP CREB binding protein [ Homo sapiens ]
• Official Symbol: CREBBP
• Synonyms: CREBBP; CREB binding protein; RSTS, Rubinstein Taybi syndrome; CREB-binding protein; CBP; KAT3A; RTS; RSTS
• Gene ID: 1387
• mRNA Refseq: NM_001079846
• Protein Refseq: NP_001073315
• MIM: 600140
• UniProt ID: Q92793

Reviews

04/17/2021

The product's adaptability to custom protocols empowered us to explore innovative experimental setups.

01/29/2019

The reduced environmental impact of the product was a responsible choice that resonated with our values.

11/15/2016

Customized solutions offered by the manufacturer increased the product's utility in our research.

Q&As

Why is the discovery of new CREBBP inhibitors urgent?

Discovering novel CREBBP inhibitors is crucial due to the limited number of reported inhibitors, and there is a need to enhance the activity and selectivity of existing compounds.

What is the significance of the bromodomain in CREBBP?

The bromodomain in CREBBP is linked to its involvement in cancer and other disease processes.

What does CREBBP stand for?

CREBBP stands for CREB-binding protein.

What is the role of CREBBP in cellular function?

CREBBP serves as a coactivator for the cAMP response element-binding protein (CREB) transcription factor. It plays a crucial role in gene activation.

What is the current status of CREBBP small molecule inhibitors?

Research on small molecule inhibitors of CREBBP is in its early stages, with only a few inhibitors reported. Improvements in activity and selectivity are needed for these compounds.

In which category of structural domain proteins does CREBBP belong?

CREBBP belongs to the third category of structural domain proteins, and it shares homology with its counterpart EP300.

How many structural domains does CREBBP have?

CREBBP contains a bromodomain, which is associated with the development of cancer and other diseases.