Recombinant Human creatine kinase, muscle and creatine kinase, brain Protein, His&Flag&StrepII tagged

• Cat.No.: CKM-264H
• Product Overview: Recombinant human CKM (P06732) with both flag and Strep II tag at the C-terminus and human CKB (P12277) with a His tag at the C-terminus are co-expression in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: CKM: C-Flag&StrepII CKB: C-His
• ProteinLength: CKM: 2-381aa
  CKB: 1-381aa
• Description: The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens. Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation.
• Tag: CKM: C-Flag&StrepII
  CKB: C-His
• Molecular Mass: 46 kDa and 43kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.
• GeneID 2: 1152

Gene Information

• Gene Name: CKM creatine kinase, muscle [ Homo sapiens (human) ]
• Official Symbol: CKM
• Synonyms: CKM; creatine kinase, muscle; CKMM; creatine kinase M-type; creatine kinase-M; creatine kinase M chain; M-CK
• Gene ID: 1158
• mRNA Refseq: NM_001824
• Protein Refseq: NP_001815
• MIM: 123310
• UniProt ID: P06732
• Gene Name 2: CKB creatine kinase, brain [ Homo sapiens ]
• Official Symbol 2: CKB
• Synonyms 2: CKB; creatine kinase, brain; CKBB; creatine kinase B-type; creatine kinase-B; creatine kinase B chain; B-CK
• mRNA Refseq 2: NM_001823
• Protein Refseq 2: NP_001814
• MIM 2: 123280
• UniProt ID 2: P12277