Recombinant Human Creatine Kinase, Mitochondrial 2 (sarcomeric), His-tagged

• Cat.No.: CKMT2-2482H
• Product Overview: Recombinant human CKMT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: CKMT2, as known as SMTCK, belongs to the ATP: guanido phosphotransferase family. CKMT2 is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. This enzyme is reversibly catalyzes the transfer of phosphate between ATP and various phosphogens.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT, 100mM NaCl.
• Molecular Mass: 46.1kDa (405aa) confirmed by MALDI-TOF
• Protein length: 40-419aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMEVREQ PRLFPPSADY PDLRKHNNCM AECLTPAIYA KLRNKVTPNG YTLDQCIQTG VDNPGHPFIK TVGMVAGDEE SYEVFADLFD PVIKLRHNGY DPRVMKHTTD LDASKITQGQ FDEHYVLSSR VRTGRSIRGL SLPPACTRAE RREVENVAIT ALEGLKGDLA GRYYKLSEMT EQDQQRLIDD HFLFDKPVSP LLTCAGMARD WPDARGIWHN YDKTFLIWIN EEDHTRVISM EKGGNMKRVF ERFCRGLKEV ERLIQERGWE FMWNERLGYI LTCPSNLGTG LRAGVHVRIP KLSKDPRFSK ILENLRLQKR GTGGVDTAAV ADVYDISNID RIGRSEVELV QIVIDGVNYL VDCEKKLERG QDIKVPPPLP QFGKK
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CKMT2 creatine kinase, mitochondrial 2 (sarcomeric) [ Homo sapiens ]
• Official Symbol: CKMT2
• Synonyms: CKMT2; creatine kinase, mitochondrial 2 (sarcomeric); creatine kinase S-type, mitochondrial; SMTCK; S-MtCK; mib-CK; basic-type mitochondrial creatine kinase; sarcomeric mitochondrial creatine kinase
• Gene ID: 1160
• mRNA Refseq: NM_001099735
• Protein Refseq: NP_001093205
• MIM: 123295
• UniProt ID: P17540
• Chromosome Location: 5
• Pathway: Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Creatine metabolism, organism-specific biosystem; Creatine pathway, organism-specific biosystem; Creatine pathway, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem
• Function: ATP binding; creatine kinase activity; nucleotide binding