Recombinant Human COPS3 293 Cell Lysate

Description :	Antigen standard for COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis) (COPS3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source :	HEK 293 cells
Species :	Human
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag :	Non

Gene Name	COPS3 COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis) [ Homo sapiens ]
Official Symbol	COPS3
Synonyms	COPS3; COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis); COP9 (constitutive photomorphogenic, Arabidopsis, homolog) subunit 3; COP9 signalosome complex subunit 3; CSN3; SGN3; signalosome subunit 3; COP9 complex subunit 3; JAB1-containing signalosome subunit 3;
Gene ID	8533
mRNA Refseq	NM_001199125
Protein Refseq	NP_001186054
MIM	604665
UniProt ID	Q9UNS2
Chromosome Location	17p11.2
Pathway	TNF-alpha/NF-kB Signaling Pathway, organism-specific biosystem;
Function	protein binding;