Recombinant Human Colony Stimulating Factor 1 (macrophage)

• Cat.No.: CSF1-5383H
• Product Overview: Recombinant HumanCSF1 was expressed in Hordeum vulgare (barley grain). Its molecular weight is21000 Dalton.

Specification

• Species: Human
• Source: Hordeum Vulgare
• Tag: Non
• Description: Recombinant humanCSF1 (Macrophage Colony Stimulating Factor) contains 158 amino acids and a 16amino acid Histidine-based tag for a total length of 174 amino acids. Thepredicted molecular weight of the recombinant M-CSF with His tag polypeptideis 20.4 kDa, but the recombinant protein migrates with an apparent molecularmass of 21 kDa in SDS-PAGE.
• Form: Lyophilized from a0.2 µm sterile filtered solution in 5 mM Tris-HCl + 80 mM Arginine, pH8.0.
• Molecular Weight: 21 kDa
• Purity: > 98% asdetermined by SDS-PAGE analysis
• Biological Activity: Determined bymeasuring the dose-dependent effect of human M-CSF on proliferation ofM-NFS-60 cells. The ED50 range is 0.51-0.76 ng/ml.
• Endotoxin Level: < 0.005 ng/µg of CSF1
• Reconstitution: Centrifuge vialprior to opening. First add sterile distilled water to the vial to fullysolubilize the protein to a concentration of 0.1-0.7 mg/ml. After completesolubilization of the protein, it can be further diluted to other aqueoussolutions.
• Storage: Store lyophilizedprotein at -20°C and reconstituted protein in working aliquots at -20°C witha carrier protein (0.1% HSA or BSA) as a stabilizer for long term storage.Please note that theaddition of any carrier protein into this product may produce unwantedendotoxin. This depends upon the particular application employed.
• OfficialSymbol: CSF1

Gene Information

• Gene Name: CSF1 colony stimulating factor 1(macrophage) [ Homo sapiens ]
• Synonyms: CSF1; colonystimulating factor 1 (macrophage); MCSF; MGC31930; colony stimulating factor1; OTTHUMP00000013363; OTTHUMP00000013364; Macrophage colony stimulatingfactor; M-CSF; CSF-1; Lanimostim
• Gene ID: 1435
• mRNA Refseq: NM_000757
• Protein Refseq: NP_000748
• MIM: 120420
• UniProt ID: P09603
• Chromosome Location: 1p21-p13
• Pathway: Cytokine-cytokinereceptor interaction; Cytokines and Inflammatory Response; Hematopoietic celllineage; Integrins in angiogenesis; Integrins in angiogenesis; Osteoclastdifferentiation; Rheumatoid arthritis; Rheumatoid arthritis; Signaling eventsmediated by PTP1B; Signaling events mediated by TCPTP
• Function: cytokine activity;growth factor activity; macrophage colony stimulating factor receptorbinding; protein binding; protein homodimerization activity