Recombinant Human COL8A2 protein, His-tagged

• Cat.No.: COL8A2-7886H
• Product Overview: Recombinant Human COL8A2 aa. (Asp537~Thr703 (Accession # P25067)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: Asp537~Thr703
• Description: This gene encodes the alpha 2 chain of type VIII collagen. This protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2. Alternative splicing results in multiple transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 19.4kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.4.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as solution form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 0.01% sarcosyl, 0.01%NaN3, and 30% glycerol.

Gene Information

• Gene Name: COL8A2 collagen type VIII alpha 2 chain [ Homo sapiens (human) ]
• Official Symbol: COL8A2
• Synonyms: COL8A2; collagen type VIII alpha 2 chain; FECD; PPCD; FECD1; PPCD2; collagen alpha-2(VIII) chain; collagen VIII, alpha-2 polypeptide; collagen, type VIII, alpha 2; endothelial collagen
• Gene ID: 1296
• mRNA Refseq: NM_001294347.1
• Protein Refseq: NP_001281276.1
• UniProt ID: P25067