Recombinant Human COL21A1 protein, His-tagged

• Cat.No.: COL21A1-72H
• Product Overview: Recombinant Human COL21A1 protein(Q96P44)(Glu23-Tyr957), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-957 a.a.
• Tag: C-His
• Form: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Molecular Mass: 97.9 KDa
• Endotoxin: < 1 EU/μg as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• AA Sequence: EDGEVRSSCRTAPTDLVFILDGSYS VGPENFEIVKKWLVNITKNFDIGPK FIQVGVVQYSDYPVL EIPLGSYDSGEHLTAAVESILYLGG NTKTGKAIQFALDYLFAKSSRFLTK IAVVLTDGKSQDDVK DAAQAARDSKITLFAIGVGSETEDA ELRAIANKPSSTYVFYVEDYIAISK IREVMKQKLCEESVC PTRIPVAARDERGFDILLGLDVNKK VKKRIQLSPKKIKGYEVTSKVDLSE LTSNVFPEGLPPSYV FVSTQRFKVKKIWDLWRILTIDGRP QIAVTLNGVDKILLFTTTSVINGSQ VVTFANPQVKTLFDE GWHQIRLLVTEQDVTLYIDDQQIEN KPLHPVLGILINGQTQIGKYSGKEE TVQFDVQKLRIYCDP EQNNRETACEIPGFNGECLNGPSDV GSTPAPCICPPGKPGLQGPKGDPGL PGNPGYPGQPGQDGK PGYQGIAGTPGVPGSPGIQGARGLP GYKGEPGRDGDKGDRGLPGFPGLHG MPGSKGEMGAKGDKG SPGFYGKKGAKGEKGNAGFPGLPGP AGEPGRHGKDGLMGSPGFKGEAGSP GAPGQDGTRGEPGIP GFPGNRGLMGQKGEIGPPGQQGKKG APGMPGLMGSNGSPGQPGTPGSKGS KGEPGIQGMPGASGL KGEPGATGSPGEPGYMGLPGIQGKK GDKGNQGEKGIQGQKGENGRQGIPG QQGIQGHHGAKGERG EKGEPGVRGAIGSKGESGVDGLMGP AGPKGQPGDPGPQGPPGLDGKPGRE FSEQFIRQVCTDVIR AQLPVLLQSGRIRNCDHCLSQHGSP GIPGPPGPIGPEGPRGLPGLPGRDG VPGLVGVPGRPGVRG LKGLPGRNGEKGSQGFGYPGEQGPP GPPGPEGPPGISKEGPPGDPGLPGK DGDHGKPGIQGQPGP PGICDPSLCFSVIARRDPFRKGPNY VDHHHHHH

Gene Information

• Gene Name: COL21A1 collagen, type XXI, alpha 1 [ Homo sapiens ]
• Official Symbol: COL21A1
• Synonyms: COL21A1; collagen, type XXI, alpha 1; collagen alpha-1(XXI) chain; alpha 1 type XXI collagen; alpha 1 chain-like collagen; FP633; COLA1L; dJ708F5.1; dJ682J15.1; FLJ39125; FLJ44623; MGC26619; DKFZp564B052
• Gene ID: 81578
• mRNA Refseq: NM_030820
• Protein Refseq: NP_110447
• MIM: 610002
• UniProt ID: Q96P44

Case Study

Case 1: Zhao Y, et al. Mol Cell Proteomics. 2024

Early pregnancy loss (EPL) often confuses diagnosis because of varied cases like hydropic abortion and different moles. To tackle this, researchers studied the proteins in pregnancy tissue. By analyzing samples from different types of EPL and normal cases, they pinpointed 14 proteins that could serve as markers. Among them, a trio of proteins—DLK1, SPTB/COL21A1, and SAR1A—show promising accuracy in identifying EPL types. They confirmed these findings with additional tests, showing strong potential for these proteins to guide better diagnosis and management of EPL.

Fig1. Representative WB bands for the expression of DLK1, SPTB, COL21A1, and SAR1A in fresh POC samples of CHM, PHM, HA, and NC.

Fig2. Basal expression of DLK1, SPTB, COL21A1, and SAR1A in two human trophoblast cell lines determined via WB.

Case 2: Fitzgerald J, et al. FEBS Lett. 2001

While digging through the human genome data, researchers found a previously unrecognized 4.1 kb cDNA encoding collagen XXI, a new member of the collagen family. This collagen has a unique structure, including a von Willebrand factor A-domain and an interrupted triple helix, fitting it into the FACIT collagen sub-family. Collagen XXI's gene, COL21A1, shows up in various tissues like the heart, stomach, and placenta and maps to chromosome location 6p11-12. These findings open new doors to understanding how this collagen functions across the body.

Fig1. MTE analysis. Probes for COL21A1 (left) and a ubiquitin control (right) were hybridized to a human MTE array.

Fig2. Northern blot analysis.

Application

Recombinant COL21A1 protein, crafted in the lab, could reshape our understanding of healing and tissue repair. This protein is a part of collagen XXI, which contributes to the structure of vital tissues like the heart and muscles. Researchers are exploring how recombinant COL21A1 might aid in fixing damaged tissues, possibly offering better ways to treat injuries or conditions that make tissues weak. It’s like finding a new formula to help the body heal more effectively. In regenerative medicine, where the goal is to rebuild or replace damaged tissues and organs, recombinant COL21A1 offers exciting possibilities. Because of its role in the extracellular matrix, scientists are interested in using this protein to create scaffolds that support new tissue growth. Such advancements could be crucial for patients needing tissue repairs or even organ transplants, providing alternative solutions and reducing dependency on donors. Moreover, beyond direct medical applications, recombinant COL21A1 could also assist in research settings. By studying this protein, scientists can get a better insight into the collagen superfamily and its various roles in the body, leading to broader implications for understanding how tissues stay healthy or succumb to diseases. Overall, this protein opens up a suite of possibilities, from patient care to scientific discovery, bringing hope and innovation to the forefront.