Recombinant Human CNTNAP1 protein, His & T7-tagged

• Cat.No.: CNTNAP1-5753H
• Product Overview: Recombinant Human CNTNAP1 aa. (Pro926~Glu1190 (Accession # P78357)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Pro926~Glu1190
• Description: The gene product was initially identified as a 190-kD protein associated with the contactin-PTPRZ1 complex. The 1,384-amino acid protein, also designated p190 or CASPR for 'contactin-associated protein,' includes an extracellular domain with several putative protein-protein interaction domains, a putative transmembrane domain, and a 74-amino acid cytoplasmic domain. Northern blot analysis showed that the gene is transcribed predominantly in brain as a transcript of 6.2 kb, with weak expression in several other tissues tested. The architecture of its extracellular domain is similar to that of neurexins, and this protein may be the signaling subunit of contactin, enabling recruitment and activation of intracellular signaling pathways in neurons.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.5kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: CNTNAP1 contactin associated protein 1 [ Homo sapiens (human) ]
• Official Symbol: CNTNAP1
• Synonyms: CNTNAP1; contactin associated protein 1; P190; CASPR; NRXN4; CNTNAP; contactin-associated protein 1; caspr1; neurexin IV; neurexin-4
• Gene ID: 8506
• mRNA Refseq: NM_003632.2
• Protein Refseq: NP_003623.1
• UniProt ID: P78357