Active Recombinant Human CLPS Protein, His-tagged

• Cat.No.: CLPS-561H
• Product Overview: Recombinant human CLPS (P04118) (Met 1-Gln 112) was fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-112 a.a.
• Predicted N Terminal: Ala 18
• Form: Lyophilized from sterile PBS, 500mM NaCl, pH 7.0, 10% gly, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.2. Immobilized human CLPS-His at 10μg/mL(100μL/well) can bind biotinylated human PNLIP-His (Cat:13564-H08H).The EC50 of biotinylated human PNLIP-His (Cat:13564-H08H) is 0.57-1.33μg/mL.
• Molecular Mass: The recombinant human CLPS consists of 105 amino acids and predicts a molecular mass of 11.5 kDa. It migrates as an approximately 12 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CLPS colipase, pancreatic [Homo sapiens]
• Official Symbol: CLPS
• Synonyms: colipase, pancreatic; colipase; pancreatic colipase preproprotein; OTTHUMP00000016271
• Gene ID: 1208
• mRNA Refseq: NM_001832
• Protein Refseq: NP_001823
• MIM: 120105
• UniProt ID: P04118
• Chromosome Location: 6pter-p21.1
• Pathway: Digestion of dietary lipid, organism-specific biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; Lipid digestion, mobilization, and transport, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Metabolism of steroid hormones and vitamins A and D, organism-specific biosystem